Labeled Nucleotides

Labeled Nucleotides

LifeSct offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable LiFluor dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA.
 

Key Features

• Bright signals with low background.

• Wide variety of fluorophores.

• Great for many applications, like Fluorescence in situ Hybridization (FISH), TUNEL, and more.
 

  Product Name Cat. # Price View
  5-Aminoallyl-dUTP (100 μl) M0070 $149
  5-Aminoallyl-UTP (100 μl) M0071 $149
  Biotin-11-dUTP (50 μl) M0072 $199
  Biotin-11-UTP (50 μl) M0073 $199
  Biotin-14-dCTP (50 nmol) M0074 $389
  Biotin-14-dATP (50 nmol) M0044 $399
  5-FAM-dUTP (25 μl) M0075 $169
  5-FAM-UTP (25 μl) M0076 $169
  5-FAM-X-UTP (25 μl) M0077 $199
  5-FAM-X-UTP (25 μl) M0078 $199
  LiFluor™ 488-X-dUTP (25 μl) M0079 $299
  LiFluor™ 488-X-UTP (25 μl) M0080 $299
  LiFluor™ 555-X-dUTP (25 μl) M0081 $299
  LiFluor™ 555-X-UTP (25 μl) M0082 $299
  LiFluor™ 568-X-dUTP (25 μl) M0083 $299
  LiFluor™ 568-X-UTP (25 μl) M0084 $299
  LiFluor™ 594-X-dUTP (25 μl) M0085 $299
  LiFluor™ 594-X-UTP (25 μl) M0086 $299
  LiFluor™ 647-X-dUTP (25 μl) M0087 $299
  LiFluor™ 647-X-UTP (25 μl) M0088 $299
  Cy3-X-dUTP (25 μl) M0142 $259
  Cy3-X-UTP (25 μl) M0143 $259
  Cy5-X-dUTP (25 μl) M0144 $259
  Cy5-X-UTP (25 μl) M0145 $259


Case Study

Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with LiFluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with LiFluor 488-X-dUTP (green) and LiFluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.

 

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with LiFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).

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