LifeSct offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable LiFluor dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA.
Key Features
• Bright signals with low background.
• Wide variety of fluorophores.
• Great for many applications, like Fluorescence in situ Hybridization (FISH), TUNEL, and more.
Case Study
Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with LiFluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with LiFluor 488-X-dUTP (green) and LiFluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.
Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with LiFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).