TGuide Smart Blood/Cell/Tissue RNA Kit adopts unique magnetic beads and a unique buffer system, to isolate and purify total RNA with high quality from blood/cell/tissue. The uniquely embedded magnetic beads have a strong affinity for nucleic acid under certain conditions. When the conditions are changed, the magnetic beads can release the adsorbed nucleic acid, to rapidly separate and purify the nucleic acid.
It can be used to perfectly fit with TGuide S16 Nucleic Acid Extractor. Through adsorption, transfer and release of magnetic beads by the special magnetic bar, magnetic beads and nucleic acid can be transferred, to improve the degree of automation. The whole process is safe and convenient, and the extracted RNA has high purity. If high throughput automated extraction is required, TIANGEN can deliver integration solutions.
RNA purified with this kit is suitable for downstream experiments including RT PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis and molecular cloning.
Key Features
• Simple and fast: Ultra-pure total RNA can be obtained by running TGuide S16 for 59 minutes .
• Flexible flux: It is perfect fit for TGuide S16 automatic Nucleic acid extraction and purification instrument to extract 1-8 samples.
• Ultra-pure: Safe and non-toxic: No toxic reagents such as phenol/chloroform.
• High purity: The RNA obtained has high purity and can be directly used for chip detection, high-throughput sequencing and other experiments.
Components
1. Buffer RL: 30 ml
2. Buffer RD: 12 ml×2
3. Blood/Cell/Tissue RNA Reagents: 48
4. Proteinase K: 1 ml
5. RNase-Free ddH2O: 15 ml
6. Buffer RDD: 4 ml
7. RNase-Free ddH2O: 1 ml
8. RNase-Free DNase I (1500 U): 1 pcs
9. TGuide Smart Tip Comb: 12 pcs
Storage
All components of the kit can be stored in dry conditions at room temperature (15-30°C) for 12 months. RNase-free DNase I, RDD buffer and RNase-free ddH2O (in tubes) can be stored at 2-8°C for 12 months.
Super easy to use
No extra pipetting work
The elution volume can be adjusted as required.
The installation and operation require little training. With pre-installed programs, unpack the cartridge, select the protocol and run your experiment. Programs are ready-to-use, and also customizable.
Preloaded reagents and matched disposable consumables.
The chemical handling is eliminated to the greatest extent to ensure the operation safety.