LifeSct provide a range of plasmid DNA purification kits to eliminate the challenges raised by multi sample processing. We design our plasmid DNA extraction products to isolate plasmid DNA at the purity and scale you need.
Selection Guide
Product Name | Features | Extraction | Yield (μg) |
TIANprep Mini Plasmid Kit | • Classic mini plasmid kit with the highest | Ordinary | Low copy: 3-12; High copy: 6-30 |
TIANprep Rapid Mini Plasmid Kit | • Convenient and rapid, 8 min quick extraction • The colorful indicator added in the buffer |
Ordinary | Low copy: 3-10; High copy: 6-24 |
EndoFree Mini Plasmid Kit | • The extracted plasmid can be used to transfect endotoxin-sensitive cells | Ultra low endotoxin residue | Low copy: 3-12; High copy: 6-30 |
TIANprep Mini Plus Plasmid Kit | • The best-selling high-purity medium volume extraction kit | High purity | Low copy: 5-25; High copy: 15-70 |
EndoFree Midi Plasmid Kit | • Ultra low endotoxin and medium volume extraction | Ultra low endotoxin residue | Low copy: 5-25; High copy: 15-70 |
EndoFree Maxi Plasmid Kit | • High-purity plasmid extraction, with the colorful indicator for lysis degree observation | High purity | Low copy: 200-600; High copy: 500-1500 |
EndoFree Maxi Plasmid Kit V2 | • Large volume extraction • The plasmid can be used to transfect endotoxin-sensitive cells |
Ultra low endotoxin residue | Low copy: 50-300; High copy: 500-1500 |
Case Study
Endofree Mini Plasmid Kit and A Company kit were used respectively. Different volumes of pAAV plasmids were extracted at the same time, the elution volume was 50 μl, 2 μl of samples were taken, the concentration of agarose gel was 1%, and electrophoresis was performed at 6 V/cm for 18 min.
pEGFP obtained with EndoFree Maxi Plasmid Kit were separately transfected into endotoxin-insensitive cell line 293T and endotoxin-sensitive cell line Huh7 cells. Expression of GFP were detected in 24 hours after transfection.
Publications
1. Dynamics of extrachromosomal circular DNA in rice.
Publication: Nature Communications Product: TIANprep Mini Plasmid Kit
2. Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae.
Publication: Cell Reports Product: TIANprep Rapid Mini Plasmid Kit
3. RGD-p21Ras-scFv expressed prokaryotically on a pilot scale inhibits ras-driven colorectal cancer growth by blocking p21Ras-GTP.
Publication: BMC Cancer Product: EndoFree Mini Plasmid Kit II
4. Abnormal upregulation of NUBP2 contributes to cancer progression in colorectal cancer.
Publication: Molecular and cellular biochemistry Product: EndoFree Maxi Plasmid Kit
5. In vivo self-assembly and delivery of VEGFR2 siRNA-encapsulated small extracellular vesicles for lung metastatic osteosarcoma therapy.
Publication: Cell death & disease Product: EndoFree Maxi Plasmid Kit V2